Development and Validation of an RP-HPLC Method for Estimation of Secnidazole and Its Degradation Products in Tablets-Juniper Publishers

Juniper Publishers-Open Access Journal of Pharmacy & Pharmaceutical Sciences


Authored by Ali Abdo Saif Ahmed Algaradi

A simple, precise, specific, sensitive and accurate stability indicating RP-HPLC method for determination of secnidazole and its degradation products in tablets. The separation was performed on teknokroma, tracer excel C18 column (25cm x 0.46cm.5μm) using mobile phase consisting of water: methanol: acetonitrile in ratio (73: 17: 10). A flow rate was set at 1mL/min; the detection wavelength was set at 228nm. The calibration curves were found to be linear in the concentration range of 50-150/mL (r2 =0.9997) and 0.25-7.5μg/mL (r2 =0.998) at assay level and low-level of secnidazole. The percentage recoveries of secnidazole were 100.76-101.66% and 97.87-102% at assay and low-level, respectively at 95% confidence limit. The intraday precision was 0.553% and 1.35% at assay level and low-level, respectively. The intermediate precision was 0.56% and 3.10% at assay level and low-level, respectively.

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